Determination of the limits for quantification of the degree of internalization of γ-Fe<sub>2</sub>O<sub>3</sub> nanoparticles by cultures of human mesenchymal stromal cells

Burban, E. A.

doi:10.31857/S0023291224060023

PII

10.31857/S0023291224060023-1

DOI

10.31857/S0023291224060023

Publication type

Article

Status

Published

Authors

E. A. Burban

Affiliation:

Volume/ Edition

Volume 86 / Issue number 6

Pages

687-699

Abstract

A culture of human bone marrow mesenchymal stromal cells (MSCs) was investigated in the present work. Cell culture was grown as a monolayer in a nutrient medium into which a stabilized aqueous suspension of magnetic nanoparticles (MNPs) of maghemite (γ-Fe₂O₃) were added. MNPs were synthesized by the electrophysical method of laser target evaporation. A method has been proposed for stabilizing a suspension in a nutrient medium with high ionic strength. A qualitative assessment of the possibility of internalization (either by fixing on the cell membrane or by penetrating into the cell space) of MNPs with human MSCs was carried out using optical, scanning and transmission electron microscopy and SQUID magnetometry. Comparative analysis of the structure and magnetic properties was made, and assumptions about the features of MNP internalization in this system were provided. It has been established that the limiting value for MNPs that can reliably be analyzed in a biological sample of the type under consideration with nanoparticles of this type is of about 0.005 mg. It was found that in the considered range of initial concentrations of magnetic nanoparticles in biological samples based on human MSCs, the level of accumulation of magnetic nanoparticles in cell cultures depends on their concentration.

Keywords

метод лазерного испарения мишени магнитные наночастицы маггемит стабилизированные водные суспензии мезенхимальные стромальные клетки человека просвечивающая электронная микроскопия магнитные измерения

Date of publication

15.11.2024

Year of publication

2024

Number of purchasers

Views

References

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GOST	Burban E. Determination of the limits for quantification of the degree of internalization of γ-Fe₂O₃ nanoparticles by cultures of human mesenchymal stromal cells // Colloid Journal. – 2024. – V. 86. – Issue number 6 C. 687-699 . URL: https://colloidras.ru/10-31857-s0023291224060023-1/?version_id=117067. DOI: 10.31857/S0023291224060023
MLA	Burban, E. A "Determination of the limits for quantification of the degree of internalization of γ-Fe₂O₃ nanoparticles by cultures of human mesenchymal stromal cells." Colloid Journal. 86.6 (2024).:687-699. DOI: 10.31857/S0023291224060023
APA	Burban E. (2024). Determination of the limits for quantification of the degree of internalization of γ-Fe₂O₃ nanoparticles by cultures of human mesenchymal stromal cells. Colloid Journal. vol. 86, no. 6, pp.687-699 DOI: 10.31857/S0023291224060023

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Determination of the limits for quantification of the degree of internalization of γ-Fe₂O₃ nanoparticles by cultures of human mesenchymal stromal cells

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Determination of the limits for quantification of the degree of internalization of γ-Fe₂O₃ nanoparticles by cultures of human mesenchymal stromal cells